WebFeb 7, 2024 · Brefeldin A was added to the cell culture medium during the last 6 h of incubation. After stimulation, the cells were collected and analyzed by flow cytometry. Flow Cytometric Analysis. Cell surface marker staining was performed on unstimulated or stimulated PBMCs. WebMar 12, 2024 · Tripartite-motif 21 (TRIM21) is thought to regulate the type I interferon (IFN) response to virus pathogens and serve as a cytosolic Fc receptor for immunoglobulin. Since herpes simplex virus (HSV)-1 is sensitive to type I IFN and neutralizing antibody, we investigated the role of TRIM21 in response to ocular HSV-1 infection in mice. In …
Evaluation of monensin and brefeldin A for flow …
Web2.1 Brefeldin A ( BFA) BFA ( Figure 1) is a macrolide antibiotic produced by diverse kinds of fungi. It was reported to inhibit protein and nucleic acid syntheses [13). We found its … WebIntracellular flow cytometry is a powerful technique for the identification of cell types and the analysis of signaling and functional responses within cell lines and heterogeneous … share network pc
Flow cytometric detection of antigen-specific cytokine ... - PubMed
WebQuadrant lines were set based on staining of Th17-polarized cells treated with brefeldin A but not PMA and ionomycin. Figure 4. Cytokine staining in mouse Th17-polarized cells. Staining of Th17-polarized, PMA-, ionomycin-, and brefeldin A–treated Balb/c splenocytes with the Invitrogen eBioscience Mouse Th17 Cytokine Staining Panel. WebApr 13, 2024 · After a total culture of 10–12 days, when cells were resting, they were re-stimulated with PMA, ionomycin, and brefeldin A as described above. Binding assays. Erythrocytes from full blood samples were lysed with Ammonium ... Cells were analyzed by flow cytometry. Flow cytometry. Cells were acquired on a FACS Canto II (BD … WebApr 22, 1999 · The two most widely used reagents are monensin and brefeldin A (BFA). These reagents differ somewhat in their mode of action, which might explain their different effects. Monensin is an inhibitor of trans-Golgi function, while BFA inhibits protein transport between the endoplasmic reticulum (ER) and the Golgi. share networking