Cspb promoter

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August undefined, 2024

WebThe major cold shock protein of Bacillus subtilis CspB binds with high affinity to the ATTGG- and CCAAT sequences in single stranded oligonucleotides ... a cis-element found in the promoter region of mammalian major histocompatability complex class II genes, which has the consensus sequence CTGATTGGC/TC/TAA [4,5]. The underlined penta- WebThe mission of the SPCB is to provide independent, verifiable evidence of proficiency in specialty pharmacy practice through a nationally recognized, accredited, and rigorous …

Direct production of L-lysine from raw corn starch by ... - PubMed

WebNov 1, 1992 · The DNA sequence of the cspB promoter region is shown at the bottom. Interruption of the chromosomal copy of the cspB gene by a double-crossover recombination event. WebThe xfp gene encoding PKT was cloned from Bifidobacterium animalis and overexpressed under the strong cspB promoter in C. glutamicum. A functional enzyme was detected in an L-Glu-producing strain of C. glutamicum (odhA). When cells of this producer strain with the xfp gene and those without the xfp gene were cultivated in a controlled ... how to stop search suggestions in edge

Cyclic AMP receptor protein (CRP) regulates the expression of

WebFurthermore, the promoter of the cspB gene was mapped by 5′ rapid amplification of cDNA ends PCR and the corresponding DNA fragment was used in DNA affinity purification … WebThe synthetic promoter library covered a wide range of strengths, ranging from 1 to 193% of the tac promoter. 68 promoters were selected and sequenced for correlation analysis between promoter ... WebJan 1, 2008 · The promoter and signal sequence of the cspB gene encoding PS2, the major protein secreted by the organism, have been used in many studies of heterologous protein secretion in C. glutamicum [43,44 ... read king james bible in one year

Characterization of the cspB gene encoding PS2, an …

Production of Chryseobacterium proteolyticum protein …

WebApr 27, 2011 · Therefore, vector pCRD314 containing tac promoter, SD sequence of tpi, and gfp gene was constructed. Second, tac promoter was replaced with promoters of PS1-encoding cspA or PS2-encoding cspB of C. glutamicum, as enhanced heterologous protein secretion was previously achieved using cspB promoter and cspA signal sequences … WebDownload scientific diagram Genetic features of the cspB promoter region of C. glutamicum ATCC 14067 and visualization by SDS-PAGE of proteins binding in front of the cspB gene. (a) The ... how to stop searching on bing in address barWebunder the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain. Immu-nostaining microscopy and flow cytometric analysis revealed that PorB-AmyA, PorC-AmyA, and PorH-AmyA were displayed on the C. glutamicum cell surface. AmyA activity was only detected in the cell fraction of C. read king of octagon

"WebA new class of cold shock-induced proteins that may be involved in an adaptive process required for cell viability at low temperatures or may function as antifreeze proteins in … " - Cspb promoter

Cspb promoter

WebCPB distributes community service grants (CSGs) to noncommercial public television and radio stations that provide significant public service programming to their communities. … WebJan 16, 2024 · The Duffer Brothers, Brittany Howard, Lyle Lovett, Qui Nguyen, Angie Thomas, Billy Bob Thornton, Mary Steenburgen and Jesmyn Ward Among Featured …

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WebFeb 6, 2012 · The latter is flanked by two loxP sites and constitutes of a transcriptional promoter (P-35S), the nptII coding sequence (CS-nptII), and a polyadenylation sequence from the nopaline synthase gene (T-nos). MON 87460 contains one copy of the insert at a single insertion site hosting both cspB and nptII intact expression cassettes. WebWe used alpha-amylase from Streptococcus bovis 148 (AmyA) as a model protein to be displayed on the C. glutamicum cell surface. AmyA was fused to the C terminus of the porins PorB, PorC, or PorH. Expression vectors using fused proteins under the control of the cspB promoter were constructed and introduced into the C. glutamicum Cm strain.

WebSep 22, 2007 · Corynebacterium glutamicum is an important microorganism in the industrial production of amino acids. We engineered a strain of C. glutamicum that secretes α … WebJan 1, 2000 · The cspB promoter (PcspB) described by Peyret et al. (1993) was isolated as a. 554 bp Eco RI \ Eco NI fragment and ligated to an Eco NI \ Bam HI adaptor constructed with the two synthetic oligo-

WebThe cspB promoter shows sequence similarity to the cspA promoter. Furthermore, both cspA and cspB mRNAs have unusually long 5′ untranslated regions (159 and 161 bases, respectively), both of which are able to form similar extensive secondary structures. These features are considered to contribute to the nature of the thermostats for cspA and cspB WebCSPB may refer to the following: California State Personnel Board, one of California's state constitutional offices; Credit Suisse Private Banking, a financial services company in …

WebDec 31, 2002 · AmyA expression under the control of cspB promoter was assumed to be induced when raw starch was used as the sole carbon source. These results indicate that efficient simultaneous saccharification and fermentation of raw corn starch to L-lysine were achieved by C. glutamicum secreting AmyA using the cspB promoter and signal …

WebJul 5, 2000 · In E. coli, CspA, CspB, CspG, and CspI are cold-shock inducible; CspC and CspE are constitutively produced at 37°C; CspD is induced at the early stationary phase ... (Left) or T metYt1 (Right) fused to the T7 A1 promoter. (C) Effects of CspA, CspB, CspC, and CspE on transcription termination at tR2. Csp proteins (CspE, CspA, ... read king of manifestationsWebJul 3, 2024 · The promoter of elongation factor Tu (tuf), which is known as a strong promoter in C. glutamicum, was amplified by PCR using primers P15 and P16 for the … how to stop searching in yahoo operaWebNational Center for Biotechnology Information read king\u0027s cage online freeWebJan 31, 2015 · In the present study, we show that cspB, cspG and cspI, the members of cspA family and induced in response to cold shock (reviewed by Gualerzi et al. 2003), … read king of battle and blood online freeWebApr 26, 2011 · The promoter of the cspB gene coding for PS2 was found to direct expression of the Mycobacterium tuberculosis gene coding for the fibronectin-binding protein 85A (Salim et al. 1997), the Streptoverticillium … how to stop searching on bingWebSep 4, 2003 · Synthesis of CspB is high when these strains are grown on lactate, acetate, gluconate and tartrate but low when the carbon sources are glucose, fructose, sucrose … read king of wrath online freeWebSep 1, 2006 · A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] production by Corynebacterium glutamicum was developed by introducing the phbCAB operon derived from Ralstonia eutropha.P(3HB) synthase activity was detected in this recombinant C. glutamicum carrying a cell surface protein gene promoter. Intracellular P(3HB) was … read king of mercinary