Fccp tom20
WebWe'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2024 ... WebOct 18, 2024 · Cell culture and treatment. Human glioblastoma cell line A172 (ATCC ® CRL 1620™) was purchased from American Type Culture Collection. These cells were cultured with L-DMEM supplemented with 10% FBS (Biowest, Mexico City, Mexico, USA) and 1% penicillin/streptomycin in a humidified atmosphere with 5% CO 2 at 37°C. Different doses …
Fccp tom20
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WebDec 12, 2024 · The expression of TOM20_GFP was confirmed in HEK293 cells using a western blotting assay with an anti-GFP antibody . To observe the localization of TOM20_GFP, HEK293 cells were stained with MitoTracker Red CMXRos, followed by a primary antibody against an anti-GFP antibody and an Alexa Fluor 488-conjugated … WebFCCP DMSO Control Rab9S21N Control Rab9S21N GFP Tom20 Merge 0 20 40 60 80 100 DMSO FCCP % of cells w. cleared mitochondria GFP GFP-Rab9S21N ***** a. 0 2 4 6 8 10 0hr 4hr 6hr # of GFP-Rab9+/Tom20+ puncta/cell 0 10 20 30 40 50 60 70 0hr 4hr 6hr # of GFP-Rab9+ puncta/cell *** **** b. c. Control Rab9 Tom20 6h FCCP Merge ns ns FCCP …
WebApr 2, 2024 · Indeed, staining with TOM20 in DBA-positive cystic epithelia showed an appreciable reduction of mitochondrial mass also at P4 in comparison to control tubules … Web首先，通过文章题目可知，e3连接酶是march5，底物分子是fundc1，tim23、tom20是线粒体膜标志蛋白（线粒体自噬过程中发生降解），表型为低氧线粒体自噬。 结合摘要我们推断出科学假设：march5通 …
WebDephosphorylationoccurspriortothereductionofFUNDC1and other mitochondrial marker proteins, such as TOM20 (an outer membrane protein) and TIM23 (an inner membrane … WebDec 28, 2024 · Cells were incubated with anti-TOM20 antibody (Santa Cruz Biotechnology, sc-17764, 1:100) in PBS containing 0.1% Triton X-100 and 1% horse serum overnight. Cells were then incubated with Alexa Fluor 488-conjugated, donkey anti-mouse IgG antibody (Jackson ImmunoResearch Laboratories, 715-546-151, 1:1000) with Hoechst 33342 …
WebPrevious studies demonstrated that mitochondrial fission arguments the stemness of bone marrow-derived mesenchymal stem cells (BMSCs). Because mitophagy is critical in removing damaged or surplus mitochondrial fragments and maintaining mitochondrial integrity, the present study was undertaken to test the hypothesis that mitophagy is …
WebOct 15, 2024 · Immunofluorescent detection of the mitochondrial outer membrane-localized protein TOM20 suggested that ATP8B1 knockdown cells had a more fragmented ... (port A), 1.1 µM FCCP (port B), and 4.62 μM antimycin A/2.31 μM rotenone (port C) were sequentially added (all chemicals from Sigma-Aldrich). Where indicated cells were … black and white nail polish meaningWebOct 1, 2024 · Cells coincubated with FCCP (6 µM during 15 min) had no TMRM fluorescence (data not shown). Oxidative stress was evaluated using MitoSox™. Briefly, cells expressing the different constructs were incubated with 5 µM MitoSox™ during 45 min at 37 °C in 5% CO₂ and 95% humidity. gagarin the first person in spaceWebTranslocase of outer mitochondrial membrane 20 (TOMM20) plays an essential role as a receptor for proteins targeted to mitochondria. TOMM20 was shown to be overexpressed … gagas 2018 pdf formatWebAug 17, 2024 · a The purity of isolated mitochondria analyzed by western blotting for HSP60 and MRPL44 (mitochondrial matrix proteins), Tom20 (mitochondrial outer membrane protein), and ERP44 (endoplasmic... black and white nail polish ideasWebOct 22, 2024 · FCCP treatment elevated the ROS production in control group, similar to the results obtained vis supplementation of erlotinib, However, Mdivi-1 application attenuated erlotinib-mediated mROS overloading (Fig. 3 a, b), indicating the necessary role that is played by mitochondrial fragmentation in mROS generation. Excessive mROS … gagas 3.110 application guidanceWebImmunoblot analysis revealed defective FCCP-induced mitophagy in Granta-519 as COXIV and Tom20 levels were not affected by FCCP treatment while they were reduced in FCCP-treated Jeko- 1 and Mino … gaga ruthenianWebInsets show typical mitochondrial morphology. ( B) mCherry-Parkin-expressing cells were treated with HBSS for 7 h, then fixed and stained with the mitochondrial marker Tom20. Representative images show mitochondrial clearance in atg5+/+ cells during starvation (reduction in Parkin- and Tom 20-positive cells). black and white nail polish designs