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WebItem Hieff Clone® Plus Multi One Step Cloning Kit; Company Yeasen Biotechnology Co., Ltd; Catalog Number 10912; This product is no longer available on Biocompare. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. WebProduct Description. Hieff Unicon TM Hotstart Direct Taq DNA Polymerase is a "hot start" DNA polymerase that tolerates blood and other repressors. This product is blocked by an …
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Web30 de set. de 2024 · Ouvir: Uso do hífen: 18 regras para entender como empregar o hífen 0:00. O hífen é um sinal ortográfico com o formato de um pequeno traço horizontal ( - ). … Web11 de fev. de 2024 · Then RNAs were reverse transcribed into cDNA by HiScript® II Q RT SuperMix for qPCR (+gDNA wiper) (Vazyme, Nanjing, China). qRT-PCR was performed using Hieff UNICON®qPCR SYBR Green MasterMix ...
Web30 de nov. de 2024 · The related primer sequences are shown in Table S1. qRT-PCR was conducted following the instructions for the Hieff UNICON® qPCR SYBR® Green Master Mix (Yeasen, Shanghai, China). The amplification system was 20 μL: Hieff UNICON® qPCR SYBR® Green Master Mix 10 μL, cDNA 1 μL, upstream and downstream primer 0.8 μL … WebHieff® qPCR SYBR Green Master Mix(No Rox)是2×实时定量PCR扩增的预混合溶液。Mix中含有热启动Hieff® DNA Polymerase、SYBR Green I、dNTPs、Mg2+。使用时, …
Web核心组分 Hieff UNICON ® Taq DNA聚合酶采用抗体法热启动,可以有效抑制样品准备过程中引物退火导致的非特异性扩增。 同时配方添加了提升PCR反应扩增效率因子和均衡不 … Web11 de fev. de 2024 · Hieff TM qPCR Mix 的优势所在! 1. 线性检测范围广 图1. 以质粒DNA(10 6-10 1 拷贝/ul)为模板,10倍梯度稀释,扩增拟南芥Actin基因。结果显示Hieff …
Web1 de jul. de 2024 · The mRNA levels were assessed by qRT-PCR with the Hieff UNICON® qPCR SYBR Green Master Mix (YEASEN, Shanghai, China) in 20 μL reactions containing 200 ng of complementary DNA (cDNA) and 100 nM of each primer using an iQ5 Optical System (Bio-Rad, Hercules, CA, USA) following the manufacturer's instructions.
Web14 de mar. de 2024 · Hieff Unicon® C58P1 nCov Multiplex One Step RT-qPCR Probe Kit is a multiplex quantitative PCR kit based on RNA as template. In the process of the experiment, reverse transcription and … cytogenetics nottinghamWeb本产品是2×实时定量PCR扩增的预混合溶液。采用的抗体法热启动DNA聚合酶(货号:10113),在预变性温度(95℃)加热30 sec,UNICON® Taq抗体失活,释放出Taq … cytogenetics nhsWebHieff Unicon™ Hotstart Direct Taq DNA Polymerase, 5 U/μL. Hieff UNICON™ Hotstart J-Taq DNA polymerase, 5 U/μL. Hieff Unicon™ Universal Multiplex qPCR Master Mix … cytogenetics multiple myelomaWebHieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) is a qPCR master mix containing a special ROX … cytogenetics miscarriagecytogenetics notesWeb1 de out. de 2024 · Total RNA of liver tissue was isolated using Trizol as previously described [28].Total RNA was transcribed using the Hifair® II 1st Strand cDNA Synthesis Kit to synthesize cDNA. cDNA samples were used for real-time PCR in a total volume of 20 μL using Hieff UNICON® Power qPCR SYBR Green Master Mix (Yeasen, China) and … bing background not changingWeb14 de mar. de 2024 · Hieff Unicon® C58P1 nCov Multiplex One Step RT-qPCR Probe Kit is a multiplex quantitative PCR kit based on RNA as template. In the process of the … bing background of the day information