Phis2.1
WebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … WebbThe promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ...
Phis2.1
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Webb24 mars 2024 · In case of 4CRM1-pHis2.1, 4CRM1mut2-pHis2.1, and 4CRM1mut5-pHis2.1, 50 mM 3-amino-1,2,4-triazole (3-AT) was sufficient to suppress background growth of yeast colonies. DNA from the TF-only prey library in pDEST_GAD424 (Mitsuda et al. 2010) was transformed into Y1HGold containing 4CRM1-pHis2.1.
Webb25 okt. 2016 · The 43-bp NWRE fragment amplified by PCR was inserted into pHIS2.1 (Clontech, CA, USA) to generate the plasmid pHIS2.1-NWRE as bait. The coding regions of IbbHLH3 and IbbHLH4 were separately cloned into pGADT7 as effectors. Lithium acetate-mediated transformation was performed to transform the constructs into yeast strain … Webb1 juni 2024 · When the insertion sequence is predicted as known cis-acting element , construct the pHIS2 reporter by three tandem copies of the studied elements inserted at …
Webb6 feb. 2024 · Europe PMC is an archive of life sciences journal literature. WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: …
Webbyeast reporter vector phis2 1 bd ( TaKaRa ) 95. TaKaRa yeast reporter vector phis2 1 bd. Yeast Reporter Vector Phis2 1 Bd, supplied by TaKaRa, used in various techniques. Bioz …
Webb15 okt. 2024 · For the protein–DNA-binding experiment, an oligonucleotide sequence containing triple tandem copies of the W-box and mW-box was synthesised, annealed, and cloned into the pHIS2.1 vector forming W-box- and mW-box-specific reporter vectors. phone number for wounded warrior projectWebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … how do you say athletic in spanishWebb12 okt. 2024 · Moreover, a yeast one-hybrid assay was performed to verify the interaction between CnGATA20 and the CnOLE18 promoter. pHIS2.1-pro53 and pGADT7-53 (pHIS2.1-pro53+pGADT7-53) were cotransferred into yeast Y187 as a positive control, and pHIS2.1-proCnOLE18+pGADT7-53 was used as a negative control. phone number for wow customer serviceWebb17 juni 2024 · Expression pattern analysis of OsbZIP62 under abiotic stresses and hormones. a, Relative expression level of OsbZIP62 under various stresses, including PEG6000 (15%), cold (4 °C), heat (42 °C), NaCl (150 mM), H 2 O 2 (1%) and ABA (100 μM). b, Main stress-related cis-acting elements in the OsbZIP62 promoter region.c, Relative … phone number for wounded warriorsWebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... phone number for wrtaWebb18 okt. 2024 · pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit (#K1617-1). phone number for world finance kpt tnWebb14 jan. 2015 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ... how do you say athlete in spanish