Phospho erk molecular weight
WebApr 30, 2024 · These data show that HA-SOS1 WT is phosphorylated in response to treatment with 1 and led us to hypothesize that active phospho-ERK is the kinase responsible for this phosphorylation event, as S1178 is at an ERK consensus SPP motif, and SPP SOS1 phosphorylation increases in parallel with elevated phospho-ERK levels ( Fig. … WebPhospho-PERK (Thr980) Antibody (MA5-15033) in WB Western blot was performed using anti-Phospho-PERK (Thr980) Monoclonal Antibody (G.305.4) (Product # MA5-15033) and a 125 kDa band corresponding to …
Phospho erk molecular weight
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WebExcitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. Target Information ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. WebPhospho-ERK is a biomarker of response to a synthetic lethal drug combination of sorafenib and MEK inhibition in liver cancer Authors Cun Wang 1 , Haojie Jin 1 , Dongmei Gao 2 , Cor Lieftink 3 , Bastiaan Evers 3 , Guangzhi Jin 4 , Zheng Xue 3 , Liqin Wang 3 , Roderick L Beijersbergen 3 , Wenxin Qin 5 , René Bernards 6 Affiliations
WebFeb 5, 2024 · ERK 1/2 Antibody (C-9) is a mouse monoclonal IgG 2a κ ERK 1/2 antibody, cited in 460 publications, provided at 200 µg/ml raised against amino acids 101-172 mapping near the N-terminus of ERK 2 of human origin ERK 1/2 Antibody (C-9) is recommended for detection of ERK 1 and ERK 2 of mouse, rat and human origin by WB, IP, IF, IHC (P) and … WebThe MAPK/ERK pathway (also known as the Ras-Raf-MEK-ERK pathway) is a chain of proteins in the cell that communicates a signal from a receptor on the surface of the cell to the DNA in the nucleus of the cell.. The signal …
WebKeep in mind that each phosphorylation adds 80Da to the protein molecular weight (Walsh 2006). 3 Probing for the total protein Using an antibody that detects the total level of your … WebKey features and details Rabbit polyclonal to PERK Suitable for: ICC/IF, WB Reacts with: Human Isotype: IgG You may also be interested in Protein Recombinant human PERK protein (ab101115) Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab205718) Primary Anti-PERK antibody [EPR19876-294] (ab229912) View more associated products Overview Product …
WebThe MEK phosphorylates and activates a mitogen-activated protein kinase (MAPK). RAF and MAPK/ERK are both serine/threonine-specific protein kinases. MEK is a serine/tyrosine/threonine kinase. In a technical sense, …
WebJun 23, 2024 · a Receptor tyrosine kinase (RTK)–RAS-induced extracellular signal-regulated kinase (ERK) activation leads to regulation through phosphorylation of a large repertoire … pope falls 2022WebNov 29, 2024 · Using immunoblot, we detected p-ERK corresponding to its molecular weight in these samples, confirming the integrity of the p-ERK phospho-epitope (Extended Data Fig. 3b). sharepoint term group vs term setWebWhat is the molecular weight of Erk? Nonphosphorylated p44/42 MAPK (Erk1/2) Control Protein: Bacterially expressed, kinase inactive, Erk2 protein serves as a negative control for Western blotting experiments. Molecular weight of MAP kinase (Erk2) protein is 42 kDa. Supplied in SDS Sample buffer. What is the molecular weight of Erk? What is p44 p42? sharepoint terminologyWebIsotype: Mouse IgG1 Immunogen: Phosphorylated Rat ERK1 (T202/Y204) Peptide Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 5 µl RRID: AB_10895978 Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide. Regulatory Status: RUO View All Formats Preparation And Storage sharepoint term store examplesWeb1. block for 1h @ room temperature (RT) with 5% BSA 2. 1:1000 Erk or p-Erk (cell signalling) in 5% BSA, for 1h @ RT and O/N @ 4'C 3. 1:5000 2nd Ab, for 1h @ RT How can I get two … pope farm conservancyWebMar 9, 2015 · Finally, pharmacological inhibition of the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) pathway in α3β1-expressing keratinocytes caused a shift towards long variant expression, while Raf-1-mediated activation of ERK in α3-null keratinocytes dramatically enhanced short variant expression, indicating a role … sharepoint terminologiespeicherWebThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete ... pope farms produce \u0026 garden center - greeley