WebApr 4, 2024 · For the phospho-ablation mutant samples (1A, 2A, and 4A), 4–12% TruPAGE Precast Gels at 180 V for 35 min were used. Total phosphorylation level was measured using Phospho-Tag Phosphoprotein Gel Stain (ABP Biosciences) or Pro-Q Diamond Phosphoprotein Gel Stain (Invitrogen), imaged using the GE Healthcare Typhoon Trio … WebMay 1, 2008 · We demonstrate that Phos-tag gel stain can be used to detect phospho-Asp in response regulators and that Phos-tag acrylamide gel electrophoresis can be used to separate phosphorylated and unphosphorylated forms of response regulator proteins. The latter method, coupled to Western blot analysis, enables detection of specific …
Phosphate-binding tag, a new tool to visualize phosphorylated
WebWe have previously reported a neutral-pH gel system buffered with Bis-Tris hydrochloride (Bis-Tris-HCl) in Zn (2+)-Phos-tag SDS-PAGE for advanced profiling of phosphoproteins … WebWB. Simple Western™ analysis of lysates (0.1 mg/mL) from C2C12 cells treated with Thapsigargin (300 nM, 2 hours) using Phospho-eIF2α (Ser51) Antibody #9721. The virtual lane view (left) shows the target band (as … irish club danbury ct
Phos-tag Gel Stain Manual
WebPhos-tag ™ SDS-PAGE can be performed to separate phosphorylated and non-phosphorylated proteins by mixing Phos-tag ™ Acrylamide with acrylamide solution to allow for polymerization to occur. User Manual. … WebPhospho-Tag™ Phosphoprotein Destain Solution is used for phosphoprotein gel stain to reduce nonspecific binding. The destain solution is prepared with sodium acetate buffer … 30 mA/gel (constant), 60 mins 5 μg/lane of α-casein from bovine milk, dephosphorylated (Cat. No. 038-23221) 1. The product contains α-casein that hasve not been dephosphorylated. While conventional SDS-PAGE will only show a single main band, Phos-tag™SDS-PAGE will show two main bands for α … See more [Electrophoresis Buffer] Tris-Glycine SDS buffer [Electrophoresis Samples] Lane1: β-casein (AP-treatment: 0 min) Lane2: β-casein (AP-treatment: 15 min) Lane3: β-casein (AP-treatment: 30 min) Lane4: β-casein (AP-treatment: … See more X-ray irradiation (5 Gy) was performed for human lung cancer cell line Lu99, and cells were collected across different time points. Cell extracts were prepared, and SDS-PAGE was performed in 13 wells using 50 μmol/L of … See more porsche performance engine builders