WebAug 1, 2004 · Known activities of the ubiquitin-selective AAA ATPase Cdc48 (p97) require one of the mutually exclusive cofactors Ufd1/Npl4 and Shp1 (p47). Whereas Ufd1/Npl4 recruits Cdc48 to ubiquitylated proteins destined for degradation by the 26S proteasome, the UBX domain protein p47 has so far been linked exclusively to nondegradative Cdc48 … Web(D) Co-IP analysis of SUMO1 conjugation and the SHP1 phosphatase substrates STAT3, TRAF6 and STING in SHP1-WT and SHP1-K127R-Mut HCT116 cells. (E) Immunoblot …
SHP1 Decreases Level of P-STAT3 (Ser727) and Inhibits …
WebSHP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 3 µg ab227503. Western blot was performed from … WebJun 23, 2024 · in the co-IP assays might be due to the disruption of weak 102 . interactions by the non-equilibrium wash steps. 103 . PD-1-ITSM strongly prefers Shp2-cSH2 over … the salon at bergdorf goodman
SH-PTP1 Antibody (D-11) SCBT - Santa Cruz …
WebNov 4, 2024 · (B) Representative immunoblots (IBs) showing the levels of SHP1 bound to mGFP-tagged PD-1 variants pulled down from the indicated coculture lysates via GFP IP. … WebSep 29, 2024 · Shp1, encoded by the gene Ptpn6, is a protein tyrosine phosphatase that transduces inhibitory signals downstream of immunoreceptors in many immune cell types. Blocking Shp1 activity represents an exciting potential immunotherapeutic strategy for the treatment of cancer, as Shp1 inhibition would be predicted to unleash both innate and … WebMar 18, 2014 · 1. Lyse your Cells. Here you gently break open your cells to make your protein accessible to the antibody. The method of lysis is important in Co-IPs. Non-detergent, low-salt lysis buffers are a popular choice for Co-IP of soluble proteins. This kind of lysis is least likely to disturb any protein interactions. trading is permitted